【摘要】：動(dòng)物膠和蛋清是古代重要彩繪文物蛋白膠結(jié)材料,對(duì)其光老化蛋白質(zhì)結(jié)構(gòu)變化的研究與探索彩繪文物病變機(jī)理和選擇科學(xué)保護(hù)材料、修復(fù)方法密切相關(guān)。以維也納藝術(shù)歷史博物館的動(dòng)物膠(骨膠、兔皮膠、鱘魚膠)和蛋清為研究對(duì)象,利用紅外光譜技術(shù),結(jié)合酰胺Ⅰ帶去卷積和高斯擬合法研究光老化過程中蛋白質(zhì)二級(jí)結(jié)構(gòu)的改變。結(jié)果表明,四種膠光老化后仍然具備蛋白質(zhì)紅外光譜的典型吸收特征。但紅外吸收峰出現(xiàn)了不同程度的位移,其中蛋清糖苷鍵明顯藍(lán)移。酰胺Ⅰ帶去卷積和高斯擬合結(jié)果顯示,蛋白膠的二級(jí)結(jié)構(gòu)占比發(fā)生了改變。其中,骨膠α-螺旋結(jié)構(gòu)占比降低22.24%,逐漸解螺旋,無規(guī)則卷曲結(jié)構(gòu)占比明顯增高(9.76%);兔皮膠和鱘魚膠α-螺旋結(jié)構(gòu)占比分別降低5.31%,4.15%,前者無規(guī)則卷曲增幅最低(6.96%),后者反而減少,推測(cè)兩者的光老化穩(wěn)定性高。蛋清α-螺旋占比降低程度最高(44.45%),無規(guī)則卷曲占比增幅極大(27.49%),光老化穩(wěn)定性最差。證實(shí)利用紅外光譜技術(shù),結(jié)合酰胺Ⅰ帶去卷積和高斯擬合法研究文物蛋白膠料二級(jí)結(jié)構(gòu)變化的方法是可行的,但其轉(zhuǎn)化機(jī)理還需后期進(jìn)一步探討。
[Abstract]:Animal glue and egg white are important ancient cultural relic protein cementing materials. The study on the changes of protein structure of photoaging is closely related to the exploration of pathological mechanism and selection of scientific protective materials and restoration methods of painted cultural relics. The animal glue (bone glue, rabbit skin gum, Sturgeon glue) and egg white of Vienna Museum of Art History were studied. The changes of secondary structure of protein during photoaging were studied by means of amide 鈪，

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