本文選題：外傷性腦損傷　切入點(diǎn)：腦紅蛋白　出處：《四川大學(xué)》2006年碩士論文　論文類型：學(xué)位論文

【摘要】：研究背景及目的：腦紅蛋白(Neuroglobin，Ngb)是一種主要位于腦內(nèi)神經(jīng)元的攜氧球白，能可逆地結(jié)合O_2。在腦缺血缺氧時(shí)，腦紅蛋白在神經(jīng)元中表達(dá)增加，作為一種內(nèi)源性神經(jīng)保護(hù)因子可保護(hù)神經(jīng)元免受缺血性損害。目前，對腦組織Ngb mRNA表達(dá)的研究主要通過采用頸動(dòng)脈阻斷的方法來建立缺血缺氧性腦損傷模型，而用外傷造成局灶性腦損傷模型來研究Ngb表達(dá)變化規(guī)律的方法，國內(nèi)外文獻(xiàn)尚未見報(bào)道。本研究采用硬膜外局部打擊的方法建立大鼠外傷性局灶性腦損傷模型，研究傷后不同時(shí)間段和不同腦損傷區(qū)細(xì)胞凋亡和Ngb在mRNA水平表達(dá)變化規(guī)律，探討腦外傷后Ngb對神經(jīng)細(xì)胞的保護(hù)作用及其機(jī)制，以期為法醫(yī)學(xué)損傷時(shí)間推斷和顱腦損傷鑒定提供一種新的指標(biāo)。 方法：健康雄性SD大鼠隨機(jī)分為正常對照組和腦損傷組。腦損傷組大鼠予木棒以70°的角度在硬膜外局部打擊的方法造成SD大鼠外傷性局灶性腦挫傷模型。 (1)檢測打擊后腦挫傷區(qū)腦血流變化情況。 (2)正常對照組直接斷頸處死大鼠，腦損傷組于傷后的不同時(shí)間段斷頸處死大鼠，，取腦組織應(yīng)用HE染色、TUNEL技術(shù)、半定量反轉(zhuǎn)錄—聚合酶鏈?zhǔn)椒磻?yīng)(半定量RT-PCR)方法和圖像分析技術(shù)對腦外傷后神經(jīng)細(xì)胞凋亡、Ngb的表達(dá)變化規(guī)律進(jìn)行研究。 結(jié)果：(1)打擊后腦損傷區(qū)血流量變化為：先短時(shí)升高，然后下降，并維持在低于正常的水平。(2)腦損傷組大鼠的腦損傷區(qū)Ngb表達(dá)在傷后15min開
[Abstract]:Background & AIM: Neuroglobinngb) is a oxygen-carrying globular white mainly located in the neurons of the brain, which can reversibly bind to O _ 2.The expression of neuroglobin in neurons increases during cerebral ischemia and hypoxia. As an endogenous neuroprotective factor, it can protect neurons from ischemic injury. At present, the expression of Ngb mRNA in brain tissue is mainly studied by carotid artery occlusion to establish the model of ischemic hypoxic brain injury. However, the method of studying the changes of Ngb expression in the model of traumatic focal brain injury has not been reported in domestic and foreign literature. In this study, the model of traumatic focal brain injury was established by using the method of epidural local strike in rats. To study the changes of apoptosis and expression of Ngb at mRNA level at different time points and different brain injury areas after injury, and to explore the protective effect of Ngb on nerve cells and its mechanism after brain injury. In order to provide a new index for forensic injury time estimation and craniocerebral injury identification. Methods: healthy male Sprague-Dawley rats were randomly divided into normal control group and brain injury group. 1) the changes of cerebral blood flow in the contusion area after striking were detected. (2) the rats in the normal control group were killed by direct cervical amputation, and the rats in the brain injury group were killed at different time points after injury. The brain tissue was taken out by HE staining and Tunel technique. Semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) and image analysis were used to study the expression of NGB in neuronal apoptosis after traumatic brain injury. Results the changes of blood flow in brain injury area after brain injury were as follows: (1) the expression of Ngb in brain injury area of rats in brain injury group was opened 15 minutes after injury, then decreased, and remained below the normal level.
【學(xué)位授予單位】：四川大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2006
【分類號(hào)】：D919

【引證文獻(xiàn)】

相關(guān)博士學(xué)位論文 前1條

1 馮興軍;腦紅蛋白對創(chuàng)傷性腦損傷的保護(hù)作用研究[D];中國人民解放軍軍醫(yī)進(jìn)修學(xué)院;2008年

本文編號(hào)：1591130