本文選題：重組雙歧桿菌-LTB疫苗 + 免疫組化��； 參考：《重慶醫(yī)科大學(xué)》2010年碩士論文

【摘要】： 雙歧桿菌(Bifidobacterium)是人類腸道內(nèi)重要的益生菌,對(duì)人體具有很好的保健功能�；陔p歧桿菌表達(dá)系統(tǒng)的重組疫苗對(duì)人體可以產(chǎn)生良好的保護(hù)作用,既能使人體產(chǎn)生抵抗病原菌的抗體,又可以調(diào)整腸道內(nèi)菌群的平衡。因此我們選用雙歧桿菌作為重組疫苗的受體菌。 產(chǎn)腸毒素大腸桿菌(ETEC)是引起腹瀉的主要致病菌。它導(dǎo)致數(shù)以萬計(jì)的人們尤其是發(fā)展中國家的嬰幼兒腹瀉,同時(shí)它也是旅行者腹瀉的最常見病原菌。因此,一個(gè)安全有效的抵抗ETEC腹瀉的疫苗對(duì)公眾健康是非常重要的。 目的:為了進(jìn)一步研究重組雙歧桿菌-LTB疫苗免疫于機(jī)體后引發(fā)的免疫反應(yīng),對(duì)免疫大鼠的抗體分泌部位進(jìn)行組織細(xì)胞定位。 方法:通過ELISA方法,間接的證明了機(jī)體產(chǎn)生了IgG抗體和IgA抗體;而通過免疫組化方法,對(duì)產(chǎn)生sIgA的組織細(xì)胞進(jìn)行進(jìn)一步的定位。 結(jié)果:免疫后的SD大鼠血清中產(chǎn)生了特異性的抗體IgG,腸道內(nèi)產(chǎn)生了特異性分泌型抗體sIgA。免疫組化的圖片顯示抗體sIgA主要是由小腸組織產(chǎn)生的,分泌的細(xì)胞為小腸絨毛固有層細(xì)胞和小腸腺細(xì)胞。隨著免疫次數(shù)的增加,抗體分泌量明顯增加,疫苗激發(fā)的免疫反應(yīng)使機(jī)體大量分泌sIgA。 結(jié)論:重組雙歧桿菌-LTB疫苗免疫大鼠后抗體sIgA分泌組織為小腸,分泌細(xì)胞為小腸絨毛固有層細(xì)胞和小腸腺細(xì)胞。
[Abstract]:Bifidobacterium (Bifidobacterium) is an important probiotic in human intestinal tract and has good health function for human body. The recombinant vaccine based on Bifidobacterium expression system can produce a good protective effect on human body, which can make the body produce antibodies against pathogenic bacteria and adjust the balance of bacteria in the intestinal tract. So we choose two pairs. Sod is the recipient of the recombinant vaccine.
Enterotoxigenic Escherichia coli (ETEC) is a major pathogen causing diarrhea. It causes tens of thousands of people, especially in developing countries, and is also the most common pathogen of traveller diarrhoea. Therefore, a safe and effective vaccine against ETEC diarrhea is very important to public health.
Objective: in order to further study the immune response induced by the recombinant Bifidobacterium -LTB Vaccine Immunized by the body, the tissue cell location of the antibody secreting parts of the immune rats was carried out.
Methods: the ELISA method was used to indirectly prove that the body produced IgG and IgA antibodies, and the tissue cells that produced sIgA were further localized by immunohistochemical method.
Results: the specific antibody IgG was produced in the serum of SD rats after immunization, and a specific secretory antibody sIgA. was produced in the intestinal tract. The antibody sIgA was produced mainly by small intestinal tissue, and the secreted cells were small intestinal villus propria cells and small intestinal gland cells. The immune response stimulated by the vaccine increases the secretion of sIgA. by the body.
Conclusion: after immunization with recombinant Bifidobacterium -LTB vaccine, the sIgA secreted tissue was small intestine, and secretory cells were small intestinal villus lamina propria cells and small intestine gland cells.
【學(xué)位授予單位】：重慶醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2010
【分類號(hào)】：R392

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