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Transplantation of vascular endothelial growth factor-modifi

發(fā)布時(shí)間:2024-07-06 23:08
  Neural stem/progenitor cell(NSC) transplantation has been shown to effectively improve neurological function in rats with hypoxic-ischemic brain damage.Vascular endothelial growth factor(VEGF) is a signaling protein that stimulates angiogenesis and improves neural regeneration.We hypothesized that transplantation of VEGF-transfected NSCs would alleviate hypoxic-ischemic brain damage in neonatal rats.We produced and transfected a recombinant lentiviral vector containing the VEGF165 gene into cult...

【文章頁數(shù)】:8 頁

【文章目錄】:
Introduction
Materials and Methods
    NSC isolation and culture
    Construction of the recombinant lentiviral vector p GC-FU-VEGF165and transfection of NSCs
    Animal experiment
    Establishment of the HIBD model
    Intracerebral transplantation
    VEGF m RNA detection
    Immunohistochemical detection of VEGF
    Histological observation
    Behavioral tests
        Radial arm maze test
        Attitudinal reflex test
    Statistical analysis
Results
    Effects of hypoxic-ischemic injury and NSC transplantation on brain VEGF m RNA and protein levels
    Effect of hypoxic-ischemic injury and NSC transplantation on brain histology
    Effects of ischemia and NSC transplantation on brain functions
        Radial arm maze test
        Attitudinal reflex test
Discussion



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