越來越多的證據(jù)表明壓力可能會導(dǎo)致頭發(fā)顏色發(fā)生變化,但其潛在機(jī)制尚不完全清楚。本研究采用雌性C57BL/6小鼠腳底電刺激結(jié)合束縛來建立慢性應(yīng)激小鼠模型,并用比色法檢測小鼠皮膚和B16F10黑色素瘤細(xì)胞中黑色素含量和酪氨酸酶活性;通過酶聯(lián)免疫吸附實驗(ELISA)測定小鼠皮膚中腫瘤壞死因子α(tumor necrosis factorα, TNF-α)、白細(xì)胞介素1β(interleukin-1β, IL-1β)和白細(xì)胞介素6 (interleukin-6, IL-6)含量;通過免疫熒光染色評估小鼠皮膚中核因子κB (nuclear factorκB, NFκB)/p65亞基的含量。結(jié)果顯示:C57BL/6小鼠在慢性應(yīng)激下由于皮膚中的毛囊黑色素細(xì)胞和酪氨酸酶活性降低,其毛皮顏色從暗色變?yōu)樽厣�。同時,慢性應(yīng)激小鼠皮膚炎癥反應(yīng)增加,表現(xiàn)為皮膚中NFκB活性和TNF-α表達(dá)增加。在體外,TNF-α以劑量依賴性方式降低B16F10黑色素瘤細(xì)胞中黑色素生成和酪氨酸酶活性。以上結(jié)果表明,慢性應(yīng)激通過降低雌性C57BL/6小鼠的毛囊黑色素細(xì)胞和酪氨酸酶活性來誘導(dǎo)皮毛顏色改變,而TNF-α可能在應(yīng)激誘導(dǎo)的...

【文章頁數(shù)】：9 頁

【文章目錄】：
1 MATERIALS AND METHODS
    1.1 Animals
    1.2 Stress protocol
    1.3 Sample harvest
    1.4 Immunohistochemistry
    1.5 Immunofluorescence
    1.6 Measurement of cytokines
    1.7 Cell culture
    1.8 Measurement of melanin contents
    1.9 Measurement of tyrosinase activity
    1.1 0 Toxicity test
    1.1 1 Statistical analysis
2 RESULTS
    2.1 Chronic stress induces fur color change and decreases melanin synthesis in mouse skin
    2.2 Chronic stress decreases follicle melanocytes and tyrosinase activity in mouse skin
    2.3 Chronic stress induces inflammatory responses in mouse skin
    2.4 TNF-αdecreases melanogenesis and tyrosinase activity in B16F10 melanoma cells
3 DISCUSSION



本文編號：4056962